The second objective was to verify if the LMN cream could prevent damage caused by oxidation or neural death in cell cultures. Cultures of the hippocampal and cortical cells were pretreated with LMN cream. After causing oxidative damage with hydrogen peroxide, which killed 40% of the cells, scientists observed that a pretreatment with LMN cream was capable of diminishing, and in some cases completely preventing, oxidative damage. The hippocampal and cortical cells were also damaged using amyloid beta (anomalous deposits of this protein are related to Alzheimer's disease). The results obtained were similar to those obtained using hydrogen peroxide.

These results demonstrate that an LMN diet is capable of inducing the generation of new cells in the adult brain, and of strengthening the neural networks which become affected with age and in neurogenerative processes such as Alzheimer's disease, as well as protecting neurons from oxidative and neural damage, two phenomena which occur at the origin of many diseases affecting the central nervous system.

In this study researchers have used different biochemical and molecular analysis techniques, with the help of specific antibodies, to detect different neuronal markers implied in the process of differentiation.

The group of researchers led by Dr Unzeta has spent years studying the effects oxidases have on oxidative stress as a factor implied in neurodegenerative disorders such as Parkinson and Alzheimer's disease, and the effects of different natural products with anti-inflammatory and antioxidant properties in different experimental models of Alzheimer's disease.

The study forms part of the CENIT project, which was awarded to La Morella Nuts in 2006 under the auspices of the INGENIO 2010 programme, with the objective of establishing methodologies for the design, evaluation and verification of functional foods which may protect against cardiovascular diseases and Alzheimer's disease. With 21.15m euros in funding and a duration of four years, the project has included the participation of 50 doctors and technicians from nine different companies, four universities (7 departments) and 2 research centres.

SOURCE Universitat Aut noma de Barcelona

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